Why do we need to use bacteria for gene cloning?

Answers

Answer 1

Answer:

It is used in the step of transformation, in order to make more plasmid DNA and consequently more gene product of interest

Explanation:

DNA cloning, as a method in molecular biology, is used for the multiplication of gene (or other piece of DNA). It usually includes steps of DNA insertion into plasmid (prokaryotic circular piece of DNA). That plasmid is than introduced into bacteria via process called transformation. Transformed bacteria can produce large amount of a product of a certain gene, because of their fast gene expression.


Related Questions

Which of the following statements correctly describes the effect a nonsense mutation would have on a gene?a) It changes an amino acid in the encoded protein.b) It alters the reading frame of the mRNA.c) It introduces a premature stop codon into the mRNA.d) It has no effect on the amino acid sequence of the encoded protein.

Answers

C. It introduces a premature stop codon

Identify the feature not unique to vertebrates.

A) vertebrae

B) backbone

C) skull

D) skeleton

Answers

The answer is skull

Answer:

Skeleton is the answer

Explanation:

Vertebrates have a skeleton made of bone and cartilage inside the body. Some invertebrates have an internal skeleton, but it is not made of bone.

Glycogen synthase is an enzyme important for making glycogen. In response to glucagon, glycogen synthase is Glycogen synthase is an enzyme important for making glycogen. In response to glucagon, glycogen synthase is 1.inactivated by removing a phosphate group (dephosphorylation). 2.activated by phosphorylation. 3.inactivated by phosphorylation. 4.activated by removing a phosphate group (dephosphorylation).

Answers

Answer:

3.inactivated by phosphorylation.

Explanation:

Glycogen synthase is an enzyme, glycosyltransferase  that is involved in the conversion of glucose into glycogen. There are two ways of the regulation of glycogen synthase:

allosteric regulation- via glucose-6-phosphate, which is activator of the enzymeby phosphorilation-it deactivates the enzyme and it is triggered by glucagon from the pancreas.

The regulation of glycogen synthase is very important for  glycogen metabolism and glucose storage.

Please select the word from the list that best fits the definition Occurs when the rotation of the Earth causes air movement to curve

Answers

Answer:

Coriolis effect

Explanation:

The rotation of the Earth is one of the climate modifiers. Considering the speed of the rotation, it is no wonder that it manages to influence the air masses and their movement in the lower atmosphere. Because of the rotation of the Earth, the air masses tend to swirl counterclockwise in the Northern Hemisphere, and clockwise in the Southern Hemisphere. This process is causing lot of large cycles to occur on both hemispheres, and it is known by the term Coriolis effect.

Easily Coriolis effect. Hopes this helps.

Organism that obtains energy directly from inorganic molecules

Answers

The answer is chemoautotroph

Describe what will happen to a wave as it goes through a hole. What do you call this?

Answers

Answer:

Explanation:

As the water waves go through the gap they spread out, this is called diffraction.

The longer the wavelength of the wave the larger the amount of diffraction.

The greatest diffraction happens when the gap size is about the same size as the wavelength.

However, some organisms get all of their genetic material from just one parent organism. When this happens, this is called _______ reproduction.

Answers

Asexual reproduction

Answer: asexual

Explanation: In asexual reproduction, all of the genes that an organism carries come from just one parent. This ordinarily happens when the parent organism simply divides to create offspring. Microscopic bacteria and yeasts undergo asexual reproduction.

Thermal energy does not depend on an object’s

Answers

Answer:

specific heat. Thermal energy depends on phase, mass, and temprature of an object.

Identify whether the following statements are true or false.

Organisms in a population must compete for resources in order to survive.

The best equipped organisms survive.

Organisms of the same species are equipped with the same survival skills.

Over time, beneficial variations of an organisms will spread through generations and gradually change the population as a whole

Answers

Answer:

They are all true besides "Organisms of the same species are equipped with the same survival skills."-that one is false.

Explanation:

TRUETRUEFALSE TRUE

ANSWER ON ED

Answer ASAP! 20 points

Answers

point D is carrying capacity.

b) point D is carrying capacity. the fill carrying capacity like is at the same height as point d therefore that is correct.

The mechanism of water reabsorption by the renal tubules is ________.

Answers

Answer:

Osmosis

Explanation:

The movement of water (passive) balances the osmotic pressure within or across the tubule walls, which maintains extracellular body fluid volume.

Many birth-control pills release a constant amount of synthetic estradiols and progesterone for 21 days. True or False

Answers

Answer:

True

Explanation:

Which of these is NOT a solution that will reduce run-off water pollution: A) Farmers switching from chemical to organic fertilizer. Eliminate B) Taking local transit instead of driving whenever you can. C) Making sure to clean pet waste up and dispose of it properly. D) Making sure your car does not leak oil by getting regular tune-ups and checks.

Answers

Answer:

B

Explanation:

Oil from gas and cars does not contribute much to run off pollution. It mostly pollutes the air as it is used.

Answer:

its B i got it right with my usatestprep i got 100%

Explanation:

The greatest expression of the lac operon occurs when lactose levels are _____.
A) low and glucose levels are lowB) low and glucose levels are highC) high and glucose levels are lowD) high and glucose levels are high

Answers

D.

Lac operan starts at an off position until a substance like allolactos turns in on so the rna polymerase can go through

Final answer:

The maximal expression of the lac operon occurs when lactose is high and glucose is low, as this allows for increased cAMP which enhances transcription and the presence of lactose inhibits the repressor.

Explanation:

The greatest expression of the lac operon occurs when lactose levels are high and glucose levels are low. This is because a low glucose concentration results in a high level of cyclic AMP (cAMP), which, when bound to the catabolite activator protein (CAP), enhances the binding of RNA polymerase to the lac operon promoter, thus increasing transcription. Furthermore, the presence of lactose results in the production of allolactose, which acts as an inducer by binding to the lac repressor and disabling its ability to bind to the operator site, thereby preventing it from blocking transcription of the operon. Jointly, these conditions significantly derepress the lac operon, leading to its maximal expression.

the center of the earth. Order the layers as you would encounter them, from the outside in.

Answers

Crust, upper mantle, mantle, outer core, inner core

Answer:

1.) Crust

2.) Upper Mantle

3.) Mantle

4.) Outer Core

5.) Inner Core

Explanation:

How many aminoacyl-tRNA synthetase enzymes must be present in cells to properly synthesize proteins?

A., 3, one for each base in a codon
B., 4, one for each base in the mRNA
C., 20, one for each amino acid
D., 64, one for each possible codon

Answers

Answer:

C. 20, one for each amino acid

Explanation:

Aminoacyl-tRNA synthetase is an enzyme involved in protein synthesis (translation) with the role to attach suitable amino acid on its tRNA (amino acid that correspond to the anticodon on tRNA). As a result of its action, aminoacyl-tRNA is formed. There are the 20 different types of aminoacyl-tRNA synthethases that  make 20 different aminoacyl-tRNA one for each amino acid of the genetic code.

Tenzin, a 35-year-old female, is 5 feet, 8 inches and weighs 250 pounds. she works, goes to school, and has a 6-month-old baby. she has very little time to cook meals at home or to exercise. tenzin's mother had gestational diabetes when she was pregnant with tenzin, and tenzin had the same diagnosis with her recent pregnancy. because of this, tenzin is being monitored for the development of diabetes.

Answers

What is the question though?

Answer:

Are you asking about what the chances of her having diabetes or her child having diabetes? If so:

Explanation:

It matters on the family history. If her mother had it, and now she developed this in pregnancy, then it's heredity. The chances of her diabetes forming in her child is higher if the child is a girl and becomes pregnant, than if it was a boy.

If old mountains are worn down over the course of millions of years, what is one of the primary reasons that mountains still exist?

A.

New mountains are created through tectonic and volcanic activity.


B.

New mountains are created from decomposition of plants and animals.


C.

New mountains are created from leaf litter.


D.

New mountains are created through city development.

Answers

A because tectonic and volcanic activity is how mountains are created. Also, the other options don’t make any sense for this question.

(50 POINTS) BEST ANSWER GETS BRAINLIEST!!!
Is water a renewable or non-renewable resource?

A.Clean water enters the Earth system from rainfall and replenishes much faster than it is used, so it's renewable.

B.Water can be filtered for reuse, and cycles through Earth in the hydrologic cycle, so it is renewable.

C. There is so much fresh clean water on Earth that humans will never be able to use all of it, so it is non-renewable.

D.We cannot clean water effectively when it is polluted, so it is completely non-renewable.

Answers

B what we can be filtered for reuse, and cycles through earth in hydrologic cycle, so it is renewable

Because, water is recycled through this process it is neither gained or lost because it reuses the water to become renewable and humans and use a filter to clean water with specific chemicals that don’t harm humans and take all the pathogen or “germs” away to be able to drink safely for humans.

Answer: B

Explanation:

What can still be cleaned after being polluted as we today and while water does come down through rainfall it goes through a whole cycle. Also remember that matter can not be created or destroyed but it changes forms

Centromeres of sister chromatids disjoin and chromatids separate. Which phase does this event happen?

Answers

Answer:

Anaphase

Explanation:

Cohesin is a protein that holds sisters chromatids together. Anaphase begins with the cleavage of that protein, enabling centromeres to split, and the movement of sister chromatides (new daughter chromosomes) toward the poles.  

During the anaphase, microtubules that are not attached to chromosomes elongate and push apart. This elongation makes the cell longer and separates the poles.

Which of the following would most likely increase the rate of photosynthesis?

A. Changing a plant's light source to a lower brightness
B. Decrease the availability of water to the plant
C. Decreasing the temperature within the plant's environment
D. Exposing a plant to a higher light intensity

Answers

Answer:

D. Exposing a plant to a higher light intensity

Explanation:

Increase in light intensity serves to increase the rate of photosynthesis when other factors are present in adequate amount. It is due to the fact that the first phase of photosynthesis includes light dependents reactions that results in synthesis of ATP and NADPH in light dependent manner. These two products serve in dark phase of photosynthesis. Therefore, increase in light intensity increases the rate of photosynthesis.

Final answer:

Option D, exposing a plant to a higher light intensity, is most likely to increase the rate of photosynthesis as light provides the necessary energy for the photosynthesis reaction to occur. Other factors like water availability and temperature also influence photosynthesis but in this case, their decrease could potentially slow down the process.

Explanation:

In terms of photosynthesis, the process through which green plants and some other organisms convert light energy, usually from the Sun, into chemical energy for their growth and survival, one of the key ingredients is light. The intensity of this light can greatly affect the rate of photosynthesis. So, among the options provided,

D. Exposing a plant to higher light intensity

is likely to increase the rate of photosynthesis. This is because light provides the energy required for the photosynthesis reaction to occur.

Conversely, decreasing light intensity (option A), decreasing the availability of water (option B), or lowering environmental temperature (option C) could potentially slow down the process. For instance, in low light conditions or shade, a seedling may not have enough light to promote sufficient photosynthesis. The rate of photosynthesis can also fall when water or optimal temperature levels are inadequate, as these conditions may lead to the closure of stomata, resulting in a constrained intake of carbon dioxide, a key reactant in photosynthesis.

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How is it possible that regions thousands of base pairs from a promoter can impact transcription of that gene?
A. DNA is capable of looping, bringing distant regions in the same proximity.B. Proteins can be very large molecules, much larger than a nucleotide.C. Transcription factors work in complexes, creating large chains of proteins.

Answers

Answer:

A. DNA is capable of looping, bringing distant regions in the same proximity.

Explanation:

Transcriptional factors (such as activators or repressors) are proteins that regulate gene expression by binding to DNA sequence (such as enhancers and silencers). Consequently, gene transcription might be turned on or off. Usually binding sites for transcriptional factors are located near the promoter (initiation of transcription). But when they are located far from the gene they regulate DNA, flexibility plays a role. DNA can form loops which bring together binding sites and transcription factors.

Which of the following statements is false? Please choose the correct answer from the following choices, and then select the submit answer button. Internal fertilization occurs only in terrestrial animals. External fertilization occurs outside of an animal's body, whereas internal fertilization takes place inside an animal's body. External fertilization usually involves the production of large numbers of gametes to increase the chances of successful fertilization. Internal fertilization prevents the gametes from drying out. None of the answer options is false.

Answers

Answer:

Internal fertilization only occurs in terrestrial animals.

Explanation:

This can happen in fish which are not terrestrial animals.

Final answer:

The false statement is that internal fertilization only occurs in terrestrial animals. Internal fertilization is also seen in some aquatic species. Both internal and external fertilization have their unique advantages and are used by different species accordingly.

Explanation:

The statement claiming that internal fertilization occurs only in terrestrial animals is false. As per the given information, internal fertilization not only occurs in terrestrial animals but some aquatic animals also utilize this reproductive method. This process may take place either through direct mating or by the male depositing sperm in the environment in a protective structure that a female then picks up and integrates into her reproductive tract.

There are examples of internal fertilization in several types of aquatic species, such as some bony fish and cartilaginous fish. Furthermore, internal fertilization enables the protection of the fertilized egg from dehydration on land, and a better survival rate compared to external fertilization due to the isolation within the female's body limiting predation on young.

While it is true that external fertilization often involves the production of copious amounts of gametes to increase the chances of successful fertilization, both types of fertilization have their unique benefits and are utilized by different species based on their respective living conditions and survival strategies.

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​danielle's family is justifiably worried that her excessive use of a "downer" will lead to an accidental overdose and death. it further worries them that she often takes the drug with alcohol. which drug are they probably concerned about?

a. ​marijuana

b. ​amphetamine

c. ​phencyclidine

d. ​barbiturate

Answers

I believe it’s a or c

The nonfunctional pelvis and hind limbs found in some snakes support which statement about evolution?



a) Snakes began to evolve these structures, but selection pressures did not favor them.



b) Mutations tend to make species evolve to resemble other species in their environment.



c) Snakes evolved from the same common ancestor as species in which these structures are functional.



d) Selection pressures promoting snake evolution ceased before snake anatomy was well adapted.

Answers

Answer:

The answer is C. Snakes evolved from the same common ancestor as species in which these structures are functional.

Explanation:

The organs which apparently have no functional role in an organism but thought to be functional in past are called Vestigial Organs. One common example is the non functional Pelvis and hind Limbs in snakes. It is thought that snakes have evolved from reptiles. From fossil record it was predicted that many snakes like species were present in past and they had the pelvic bones while due to adaptation to the changing environment they lost the functional bones and evolved as snakes.  

The growing polypeptide chain is inserted into the membrane of the blank before being sent to its final destination

Answers

endoplasmic reticulum

URGENT PLZ HELP!!!!(70 POINTS!!!!) WILL MARK BRAINLIEST
I have to do this alternate assignment for Bio and i have no idea what to do, plz help.]
So i had to choose 8 pieces of furniture in my house and categorize it into "Kingdoms" and i did that, so now i have to categorize them into 4 diff phylums.
The chosen furnitures were:

computer desk, Tv, Home phone, PS4, books,Dining Table,Chair,Bed,Couch.

Answers

Answer:

Explanation:

Im pretty sure you just have to mae it into smaller categorys for exaple the home phone tv and ps4 wouuld be in 1 phylum and that phylum would be electronics and then it would be like that so forth and so forth.

Which of the following sequences of events correctly describes the progress of a protein that will be secreted from the cell?(A) SRP binds to the growing polypeptide chain and to the ribosome.(B) Translation resumes.(C) SRP binds to its receptor.(D) The signal sequence is cleaved.(E) Protein synthesis begins in the cytosol.(F) Translation pauses.•E → A → F → C → B → D•E → F → A → C → B → D•C → F → A → B → D → E

Answers

Answer:

E - F - A - C - B - D

Explanation:

After the export of mRNA from the nucleus, translation (protein synthesis) begins in the cytosol. Proteins that should be secreted from the cell, must e targeted to the endoplasmic reticulum (ER) and this process occurs co-translational (while translation is still in progress).The signal recognition particle (SRP) is involved in the process of targeting, because it recognizes specific signal sequences in proteins and targets that protein to the endoplasmic reticulum (SRP binds to its receptor in the membrane of the ER).

Biomes on Earth have exhibited changes throughout history that have been caused by a variety of factors. Over the past 50 years, the amount of tropical rainforest acreage found throughout the world has decreased from 14.8 billion acres to 8.6 billion acres due to deforestation. What factor is likely most responsible for the changes observed in the world’s rainforests?

a.volcanic activity
b.human activity
c.temperature changes
d.solar eclipses

Answers

Answer:

b.human activity

Explanation:

Deforestation is a human-driven process of the tree removal. Destruction of forest is  performed fro many reasons: urbanization of the land, industry, farms, wood and paper production etc. It increased over the past 50 years and the main target are tropical rainforests. The trees are burned or cut by humans who leave the destroyed ecosystem behind.

Deforestation affect the animals and plants that lived there, but also humans and the effect even have global consequence (global water cycles, warming).

Final answer:

The most responsible factor for the changes observed in the world's rainforests, particularly the decrease in acreage, is human activity, specifically deforestation. Deforestation, driven by agriculture and logging, has resulted in habitat destruction, loss of biodiversity, and increased carbon dioxide emissions. Conservation efforts and international agreements are being implemented to address this issue.

Explanation:

The factor that is most responsible for the changes observed in the world's rainforests is human activity, particularly deforestation. Deforestation refers to the clearing of forests, often for agriculture, logging, or urbanization purposes. In the case of tropical rainforests, human activities such as logging and slash-and-burn agriculture have contributed significantly to the loss of acreage.

For example, in the Amazon rainforest, one of the largest tropical rainforests, deforestation has been driven by the expansion of cattle ranching and soybean farming. Trees are cut down to make space for grazing pastures or to plant soybeans. This widespread deforestation has resulted in habitat destruction, loss of biodiversity, and increased carbon dioxide emissions, contributing to climate change.

To mitigate and address the issue of deforestation, conservation efforts, sustainable land management practices, and international agreements such as the REDD+ program have been implemented to promote the preservation of tropical rainforests.

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which statement is true of this poem?
a. the sea is personified as a female
b. each said that begins with alliteration
c. the Moon is personified as an old man
d. the sea is an idiom for reckless violence ​

Answers

Answer:

wrong subject but okay. The answer is (C). why?

Explanation:

Because the moon IS personified as an old man, the moon has been around for millions of years, therefore making the moon old, get it? Also post this on english or writing, not biology.

Hope you have a good day! Byeee <3333

Answer:

Its C I just did it that girl is wrong Im right.... I AM!

Explanation:

The sea is personified as a female.:333333

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