Chemistry College

Plasmid DNA and a gene of interest are cut with the enzyme PpuMI. Write a possible sequence of bases for the sticky end of the gene in the 5' to 3' direction.

Answers

Answer 1

The possible 5' to 3' sequence of bases for the sticky end of the gene cut by PpuMI is: 5'-ACGGA-3'.

The enzyme PpuMI cuts DNA at recognition sequences. In the 5' to 3' direction, "ACGGA." might be the sticky end of the gene snipped by PpuMI. This indicates that one DNA strand reads 5'-ACGGA-3' and the other 3'-TGCCT-5'.

These overhanging single-stranded ends are called "sticky ends" because they can base-pair with complementary sequences in another DNA fragment cut with the same enzyme. In genetic engineering, like cloning, DNA fragments with matching sticky ends can be joined to form recombinant DNA. PpuMI-generated sticky ends with the sequence "ACGGA" allow scientists to insert genes of interest into plasmids or other DNA molecules for study and practical applications.

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Answer 2
Final answer:

The possible sequence of bases for the sticky end of the gene in the 5' to 3' direction after being cut with PpuMI is 5'-GAAC (gene) TTGC-3'.

Explanation:

The sequence of bases for the sticky end of the gene in the 5' to 3' direction after being cut with the enzyme PpuMI can be determined from the given information. The restriction enzyme PpuMI leaves a 2- to 4-nucleotide single-stranded overhang on each strand of the DNA after cutting. The sequence that is recognized by PpuMI is a palindrome, meaning it reads the same forward and backward. Therefore, the possible sequence of bases for the sticky end of the gene in the 5' to 3' direction is: 5'-GAAC (gene) TTGC-3'

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