The "scissors" of the molecular biology are: DNA Restriction enzymes.
A restriction enzyme is a protein capable of cleaving a DNA fragment at a characteristic nucleotide sequence called a restriction site. Each restriction enzyme thus recognizes a specific site. Several hundred restriction enzymes are currently known.
Naturally present in a large number of species of bacteria, these enzymes have become important tools in genetic engineering.
The "glue" of the molecular biology are: DNA ligase
In molecular biology, DNA ligases are ligase-class enzymes that catalyze the formation of a phosphodiester bond between two segments of DNA. DNA ligases are involved in several essential cellular processes of DNA metabolism: in DNA replication, suture of Okazaki fragments, and in DNA repair and homologous recombination.
The use of these tools in molecular biology: Cloning
Molecular cloning is one of the bases of genetic engineering. It consists of inserting a DNA fragment (called insert) in an appropriate vector such as a plasmid for example. The new plasmid thus created will then be introduced into a host cell, generally the Escherichia coli bacterium. This will then be selected and multiplied to obtain a large amount of the plasmid of interest. Cloning a gene involves inserting it into a plasmid. A clone will be the bacterial transformant that contains this particular plasmid. In this case we speak of clone because all the individuals of the bacterial colony are genetically identical. Molecular cloning is thus different from reproductive cloning (creating an individual genetically identical to another but of a different age) or therapeutic cloning (making tissues from stem cells to perform transplants compatible with the recipient).
Molecular cloning requires restriction enzymes capable of cleaving the DNA, and DNA ligase capable of re-gluing the DNA fragments. Ligase was isolated for the first time from T4 bacteriophage. This enzyme is involved in the repair and replication of DNA. It can bind DNA fragments with compatible sticky ends. At higher concentration, this enzyme is also able to bind two ends of DNA as shown here. T4 DNA ligase works using ATP and Mg ++. It has an activity optimum of 16 ° C, but remains active at room temperature.
The term scissors is used in molecular biology for an enzyme which is known as DNA restriction enzyme. The term glue is used in molecular biology for an enzyme which is known as DNA ligase. These enzymes are used for cutting and sticking the DNA during recombinant DNA technology.
Further Explanation:
Restrictionenzymes commonly known as the scissors in the molecular biology are the enzymes that are used to cut the DNA into smaller parts for cut the DNA from a target sequence. There are enzymes that act as molecular scissors and cut the DNA with overhanging strands. These strands are known as sticky ends. There are also the enzymes that cut the DNA with blunt ends and these ads are known as blunt ends. Restriction enzymes predominantly used in a molecular biology or recombinant DNA technology lab. EcoR1 is the most common example of a restriction enzyme.
DNA ligase commonly known as the molecular glue is used to stick the strands of DNA that are cut by the help of restriction enzymes. A single piece of DNA is formed by sealing the gap between the molecules which is only possible with the help of DNAligase.
Both these enzymes are widely used in the recombinant DNA technology laboratory for the cutting of specific part of DNA and the insertion of intended genes into the plasmid during DNA cloning.
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Answer Details:
Grade: High School
Subject: Biology
Chapter: Molecular Biology
Keywords:
Molecular scissors, molecular glue, restriction enzyme, DNA ligase, DNA strand, plasmid, DNA cloning, recognition sites, sticky ends, blunt ends.
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Which often results from great variations in a habitat’s abiotic conditions?
increased moisture
more niches
fewer organisms
lower temperatures
how do organisms get the energy they need?
A. Plants get energy from fertilizers, and animals get energy by drinking water.
B. Plants get energy from glucose produced in the soil, and animals get energy directly from sunlight.
C. Plants do not require energy because they do not move, and animals get energy by performing exercise.
D. Plants break down the glucose they produced in photosynthesis, and animals get energy from eating other organisms.
In this Energy Acquisition question, Plants create energy by photosynthesizing sunlight into glucose, which they then break down for use. Animals get energy from eating other organisms, breaking down the food in their body to release the energy stored in it. Correct option is option D.
Organisms get the energy they need in different ways. Plants, being autotrophs, capture energy from the sun through photosynthesis to convert carbon dioxide and water into glucose, a type of sugar, which they break down to release energy as needed.
This process involves converting light energy into chemical energy stored in glucose.
On the other hand, animals, being heterotrophs, get their energy directly from eating other organisms, whether they are plants, animals, or both.
The food they consume is broken down through the process of digestion, and the nutrients, especially glucose, are used in cellular respiration to release energy essential for the various activities and functions of the body.
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How might you alter a trna so that it inserts an alanine into a polypeptide for which a lysine is coded?
__________ is the conversion of acetyl coa into ketone bodies.
Final answer:
Ketogenesis is the conversion of surplus acetyl CoA into ketone bodies, such as acetoacetate, β-hydroxybutyrate, and acetone, when the Krebs cycle is overwhelmed. This process provides an alternative energy source for the body during periods of low glucose availability.
Explanation:
Ketogenesis is the conversion of acetyl CoA into ketone bodies. When there is an excess of acetyl CoA from the oxidation of fatty acids, and the Krebs cycle (citric acid cycle) cannot process it all, the liver converts this surplus acetyl CoA into ketone bodies through a pathway known as ketogenesis. The main ketone bodies produced are acetoacetate, β-hydroxybutyrate (B-hydroxybutyrate), and acetone. The process starts with two acetyl CoA molecules combining to form acetoacetyl-CoA. This is then converted into β-hydroxy-β-methylglutaryl-CoA (HMG CoA), which subsequently gets cleaved to form acetoacetate and acetyl CoA. Most of the acetoacetate is further reduced to β-hydroxybutyrate, and a smaller portion decarboxylates to form acetone and carbon dioxide.
Ketone bodies are useful as an alternative energy source when glucose is scarce, such as during prolonged fasting or uncontrolled diabetes. They can be transported from the liver to other tissues, where they are reconverted to acetyl CoA and enter the Krebs cycle to produce energy. This ensures organs like the brain can function even when glucose supply is low.
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Permafrost remains throughout the summer because it is insulated by _____.
groundcover vegetation
thick sheets of ice
snow
topsoil
If I am correct, permafrost may last through the summer because of groundcover vegetation
Answer: groundcover vegetation
Explanation:
Compare and contrast the lowry and bradford protein assays with regard to compatibility with various potential contaminating compounds.
Which is not a use for fossils found in sedimentary rock
Determining the mineral composition of the rocks
what are the two most abundant gases in the atmosphere
Answer:
The correct answer will be- Oxygen (21%) and Nitrogen (78%)
Explanation:
The atmosphere of Earth is composed of a mixture of the gases in different concentrations.
The major constituent gases of the atmosphere are the nitrogen which constitutes about 78% of the total gases present and the oxygen which constitutes the 21% of the total gases.
These gases exist in their divalent states in the atmosphere where the nitrogen is used by the organisms when it is fixed by the prokaryotic nitrogen-fixing bacteria whereas the oxygen is directly used by the organism.
Thus, Oxygen (21%) and Nitrogen (78%) is the correct answer.
Which statement accurately describes DNA?
A. DNA is found on genes.
B. DNA is replicated by mRNA.
C. DNA is a molecule that contains genes.
D. DNA is a molecule made up of chromosomes.
Your textbook describes a field experiment using guppies that was conducted to test natural selection. complete the sentences about this experiment with the correct terms.
John Endler's field experiment with Trinidad guppies demonstrated how selection pressure and genetic variability drive natural selection in guppy populations. The shift in guppy coloration in different environments highlights the tradeoff between sexual selection and predation risk. These factors all play a role in the stability of guppy populations and ecosystems.
Explanation:The guppy field experiment conducted by John Endler in Trinidad serves as a strong example of selection pressure affecting genetic variability and natural selection. By relocating drab-colored guppies from an area with predators (area 3) to an area without predators (area 1), it was observed that, over time, these guppies developed brighter colors. This change suggests that genetic variability did exist in the original guppy population, as there were alleles present for both bright and drab coloration traits. The presence of predators in the original habitat (area 3) creates selection pressure for more camouflaged, drab colors to avoid predation, whereas, in the absence of predators (area 1 after relocation), sexual selection favored brighter colors which are more attractive to female guppies.
If guppies from a predator-free environment (area 2) were moved back to the predator-filled environment (area 3), it can be predicted that over time, the bright colors would diminish as predators would more easily catch the more conspicuous guppies. Furthermore, if female preferences shifted from coloration to size, we would expect the selective pressure to shift accordingly, and larger fish, regardless of their color, would become more prevalent in the population. This sort of selective breeding would also affect the stability of the guppy population and potentially the ecosystem, as different traits become advantageous or disadvantageous depending on the environment and predation pressure.
How does mitosis in plant cells differ from that in animal cells? A. Plant cells lack a cell membrane. B. Plant cells lack spindle fibers. C. Animal cells lack a cell plate. D. Animal cells lack cytokinesis
Plant cells have cell walls and chloroplasts, while animal cells lack these structures. Plant cells form a cell plate during mitosis, while animal cells do not.
Explanation:Plant cells and animal cells have several differences when it comes to mitosis. One major difference is that plant cells have a cell wall, while animal cells do not. This means that during mitosis, plant cells form a cell plate, which eventually becomes a new cell wall, while animal cells do not have this structure.
Another difference is that plant cells have chloroplasts, which are responsible for photosynthesis, while animal cells do not. Additionally, plant cells have plasmodesmata, which are channels that allow for communication between neighboring cells, while animal cells lack this structure.
How does a growing embryo inside a seed obtain food?
1) It uses food stored within the seed.
2) It undergoes photosynthesis to make its own food.
3) It absorbs food from the outside environment.
4) It takes in food provided by the seed coat.
Answer:
1) it uses food stored within the seed.
Explanation:
just got it right...
A developing embryo inside a seed uses food stored inside the seed to gain nutrition. So, the correct option is A.
What is an Embryo?The embryo is created shortly after fertilization when the zygote divides multiple times in accordance with the patterns unique to each species. An embryo with differentiated radicle, plumule, and cotyledons is enclosed in the seed coat.
The plumule produces the aerial shoot, whereas the radicle, when it becomes longer, produces the major root. There may be one cotyledon (monocotyledons) or two cotyledons (as in dicotyledons).
Sometimes they act as food reserves, storing grains, peas, almonds, cashews, and other foods, or they act as photosynthetic cells in immature seedlings. The hypocotyl is the portion of an embryonic axis that lies between both the radicle and the cotyledon's attachment point. In a similar vein, the region of a embryonic axis between both the plumule as well as the point of attachment of cotyledons is called epicotyl.
Thus, the correct option is A.
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In biology class one day, Mrs. Chabon wrote six physiological functions on the board. Then, she asked groups of students to each choose the three functions that are purposes of the excretory system. These are the lists they made. Group 1: maintaining pH level of blood; transporting hormones throughout the body; regulating growth Group 2: removing wastes from the body; digesting solid foods; maintaining salt and water balance Group 3: maintaining salt and water balance; maintaining pH level of blood; regulating growth Group 4: removing wastes from the body; maintaining pH level of blood; maintaining salt and water balance Which group correctly listed the functions of the excretory system? group 1 group 2 group 3 group 4
Which of the following plants do NOT have swimming sperm?
ginkgos
cycads
both a and b
none of the above
Answer:
None of these.
Explanation: The plants like cycads and ginkgos have swimming sperms. they need water to complete their life cycle.
There are many adaptations seen in the plants like liveworts, ferns and mosses in which they require water to complete its life cycle. This adaptation is not only restricted upto cyptograms. This can also be seen in the plants like ginkgos and cycads. Their sperms are very strange.
You have examined several body systems that rely and work with each other in order function properly. One very important task that amphibians and all living things need is to efficiently exchange gases in order to survive. Explain in a brief essay how the cardiovascular system and respiratory system work with each other based on your experience and observations from this virtual lab.
Complete the description of experimental evidence that would be consistent with these conclusions. drag the terms on the left to the appropriate blanks on the right to complete the sentences. terms can be used once, more than once, or not at all.
7. A system of stars orbiting a common center of gravity
A) solar system
B) universe
C) galaxy
D) biosphere
8. The group of eight planets and other matter that orbits the sun.
A) solar system
B) universe
C) galaxy
D) biosphere
9. Everything in outer space including stars,planets, and galaxies
A) solar system
B) universe
C) galaxy
D) biosphere
10. A planet that sustains life in its biosphere
A) mars
B) Venus
C) earth
D) Jupiter
What would happen to the proton gradient and atp production after a drug has poisoned the enzyme that combines acetyl coa and oxaloacetate to form citrate? see section 9.5 ( page 201) ?
A common way by which virus vaccines are made is to introduce a mutation that makes the virus less virulent (i.e. that reduces the severity of infection) and to expose patients to that mutated virus. which sort of mutation would it be safest to introduce to ensure that the virus cannot easily revert to its original, virulent state?
In an attempt to deal with his severe, untreatable epileptic seizures, h.m. had portions of his _____, including the brain structure called the _____ surgically removed.
The movement of the arms and legs is accomplished by the _____ subdivision of the peripheral nervous system.
The somatic subdivision of the peripheral nervous system controls voluntary movements like walking or moving arms.
The movement of the arms and legs is accomplished by the somatic subdivision of the peripheral nervous system.
The somatic nervous system is responsible for controlling voluntary activities, such as walking or moving your arms. It allows individuals to have direct control over skeletal muscles, enabling purposeful movement.
one hypothesized mechanism by which antidepressant medications help to regulate mood is the suppression of ______ sleep.
The first stage of catabolic metabolism occurs in the
A. gallbladder.
B. stomach.
C. pancreas.
D. liver.
Discuss the term "survival of the fittest" was developed and what it means.
Survival of the fittest is a word coined best known by British naturalist Charles Darwin in the fifth edition of On the Origin of Species, which proposed that organisms that are best adapted to their environment are the most successful in surviving and reproducing.
What is evolution?Change in the heritable traits of biological populations over successive generations is referred to as evolution. These traits are gene expressions that are passed down from parent to offspring during reproduction.
The phrase "survival of the fittest" refers to the process of natural selection, a mechanism that drives evolutionary change.
Natural selection works by favoring individuals who are better adapted to a given set of environmental conditions over those who are not.
In this context, evolution is both a fact and a theory. It is an undeniable fact that organisms have changed, or evolved, over the course of Earth's history.
Thus, it can be concluded that the term survival of the fittest was coined in fifth edition of On the Origin of Species.
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A garden has 80 pea plants. Of this total, 20 plants have short stems and 60 plants have tall stems. What percentage of the plants is short?
When salt is mixed with water and stirred, the salt will dissolve, forming a solution. When sand is mixed with water, the sand will not dissolve. Why will salt form a solution with water but sand will not?
A) Salt is pulled apart into two ions by the polar water molecules, and sand is not.
B) Sand forms complex crystals which dissolve much more slowly than non-crystalline salt.
C) Salt consists of only 2 atoms and is small enough to dissolve, while sand is too large.
D) Sand is much harder than salt and so it will not form a solution with water.
The correct answer is A) Salt is pulled apart into two ions by the polar water molecules, and sand is not.
This happens because salt has both positive and negative ends that are attracted by the polar molecules of water.
N the laboratory, you set up an experimental mating between a male and female fruit fly that are both heterozygous (aa) at a locus where the homozygous recessive genotype (aa) is lethal at the early embryo stage. in a large sample of 100 surviving offspring, how many do you expect to have the homozygous dominant (aa) genotype?
Schizophrenia is a disorder that is most often treated _______.
a. over an extended period of time
b. over a short period of time
c. only in its acute phase
d. primarily by the use of psychotherapy
Schizophrenia is most often treated: a. over an extended period of time
Schizophrenia can be described as a chronic mental health disorder which is usually complex and is characterized by delusions, disorganized speech and behavior, impaired cognitive ability and hallucinations.
Serious of treatment can be employed, which involves medications and psychotherapy. Generally, the treatment of schizophrenia takes no shorter than 6 years. It can extend to long period of time.
Therefore, Schizophrenia is most often treated: a. over an extended period of time
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For many species living in variable environments, sexual reproduction has proven to be a better solution for reproduction than asexual reproduction because sexual reproduction ______________ .
What were some of the earliest forms of life on Earth?
Answer:
The first life forms were (3.8 to 2.5 billion years ago), the Archean Period and the world's landmasses began to form in the Archean Period. Earth's initial life forms were bacteria, which could survive in the highly toxic atmosphere that existed during this time.
Explanation:
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Microbes, the earliest known life forms, left traces of their existence in rocks that were 3.7 billion years old.
What are microbes?Microbes are extremely minute living entities that are all around us and are invisible to the unaided eye.
They are aquatic, terrestrial, and avian organisms. Millions of these bacteria, which are also referred to as microorganisms, reside in the human body. While certain microorganisms make us ill, others are vital to our wellbeing.
A microorganism, often known as a microbe, is technically a tiny organism. The term "microbiology" refers to the study of microorganisms.
Bacteria, fungus, archaea, and protists are all types of microorganisms. Prions and viruses are not considered microbes because they are considered non-living in general.
The earliest known life forms, microbes, left behind signs of their presence in 3.7 billion year-old rocks.
Thus, microbes are some of the earliest forms of life on Earth.
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